Rupali was born in Kolkata on to a Hindu Bengali family. She is the daughter of acclaimed director Anil Ganguly. She started acting when she was 7 years old with a role in the film Saaheb (1985), following it up with an award winning performance in her father's film, Balidan (1990). Her latest TV serials include the hilarious comedy serial on Star One, Sarabhai vs Sarabhai (2004) in which she plays the character of Monisha.She has participated in dance reality show Zara Nachke Dikha, and Bigg Boss (2006) and stunt/dare reality game show, Fear Factor - Khatron Ke Khiladi.She also gave voice over in animation film, Dashavatar. In 2000, she established an advertising agency in Mumbai.She got married to her longtime boyfriend, Ashwin K Verma on February 6, 2013.